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Next, the supernatant was collected after centrifuging at 1000 rpm for 10 min at 4 C, and then measured at 532 nm with a microplate reader

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This is based on the observation that the livers of GCLM KO mice in all diet groups have high levels of active (phosphorylated) LKB1, a redox-sensitive kinase and the primary hepatic AMPK kinase 24

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Dietary intake and physical activity level To evaluate dietary habits, we employed a comprehensive 113-item Food Frequency Questionnaire (FFQ), along with an additional 127-item FFQ tailored to capture the consumption of traditional foods