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To investigate the regulatory effects of GLP-1RAs on cholesterol metabolism in hepatocytes treated with saturated free fatty acids, cells were pretreated with 400 M PA (Sigma-Aldrich) for 24 hours, followed by treatment with 100 nM exendin-4 (Sigma-Aldrich) or liraglutide (MedChemExpress) for an additional 24 hours

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