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Radioactive isotopes must be avoided during pregnancy and lactation

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A lengthened synthetic version of insulin-like growth factor 1, carrying an extra amino-acid extension and a single substitution, IGF-1 LR3 is studied as a long-acting growth factor analogue

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Sixteen hours after transfection, biotinylation was triggered by adding biotin to a final concentration of 50 M (1 mM biotin stock in PBS and stored for up to 6 months)