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For all MAX2 and At SMAX1 proteins, purification was done on KTA pure systems, first by affinity purification on HisTrap HP 5 mL (Cytiva) column, followed by StrepTrap XT 5 mL (Cytiva) column and then by size-exclusion chromatography (SEC) on HiLoad 16/600 Superdex 200 pg (Cytiva)

The eyebrows were sparse in 59% (36/61) and absent in 31% (19/61)

whereas the greatest level of t -cadinol (1.1%) produced by distilling EO from plants that have been treated with Zn at 0.5 g L 1 x GSH at 0.4 g L 1

Sustained was defined as having 2 consecutive measurements 28 days apart fulfilling the criteria

It means calibrating the speed of loss