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However, current evidence remains largely proof-of-concept, and translation requires careful evaluation of phototoxicity, cross-platform calibration, and protocol-dependent domain shift (culture media, incubator conditions, optics), so hyperspectral features are best positioned within standardized, prospectively validated multimodal pipelines rather than as stand-alone add-ons [444, 445]

Strategies include fractionated dose, chronotherapy, and real-time monitoring [238, 239]

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