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Gently aspirate by pulling back slightly on the plunger to check for blood: If blood appears , stop, withdraw, and restart in a new spot

The anionic GSH does not easily interact with anionic citrate-capped AuNPs because of electrostatic repulsion, and thus, GSH cannot induce the aggregation of citrate-capped AuNPs

Int J Hematol (2001) 73(3):2717.10.1007/Bf02981951 100 SchaeferTSSandersLKNathansD

Imaging was performed with a Zeiss Axio Observer.Z1 microscope with a Yokogawa spinning disk confocal head, Cascade IIL:512 camera, a quad-band notch dichroic mirror (405, 488, 568 and 647 nm) and 405-nm, 491-nm, 561-nm and 640-nm lasers (all 50 mW)

doi: 10.3390/cells11172726 60 XuX.-Q.XuT.JiW.WangC.RenY.XiongX.et al